Comunicación

NLRP3 PROMOTES AN EARLY INFLAMMATION TRIGGERING TYPE I COLLAGEN PRODUCTION IN MICE ACHILLES TENDON

Autores:

ALEJANDRO ELEAZAR PEÑIN FRANCH1, JOSÉ ANTONIO GARCÍA VIDAL2, MARIA PILAR ESCOLAR REINA2, MARINA CARPES RUIZ3, CARLOS MANUEL MARTINEZ CACERES3, FRANCISCO MEDINA MIRAPEIX2, PABLO PELEGRIN VIVANCOS1

Afiliaciones:

(1) CIRUGÍA DIGESTIVA, ENDOCRINA Y TRASPLANTE DE ÓRGANOS ABDOMINALES, IMIB-Arrixaca, España
(2) FISIOTERAPIA Y DISCAPACIDAD, IMIB-Arrixaca, España
(3) PLATAFORMA DE PATOLOGÍA, IMIB-Arrixaca, España

Comunicación:

Antecedentes:

Percutaneous electrolysis is an innovative physiotherapeutic technic that applies a galvanic current in the tissue and has been found to be more effective in the treatment of tendinopathies than other techniques as needling alone. The nucleotide-binding domain leucine-rich repeat containing a pyrin domain 3 (NLRP3) inflammasome is involved in physiological inflammation processes by activating caspase-1. Type I collagen is the most abundant collagen in the human body. The amount of type I and type III collagen in tendon determines the maturation of the tissue, being more mature with type I collagen increase. However, the effect of galvanic current in collagen tendon and its relation with NLRP3 inflammasome are not characterized.

Métodos:

C57BL/6, Nlrp3-/-, Casp1/11-/-, and Pyrin-/- mice were treated with needling and galvanic current on the Achilles tendon using 3 pulses of 3 mA for 3 seconds each. Tissue samples obtained at different days after treatments were used for haematoxylin and eosin staining, Sirius red staining and F4/80 immunohistochemistry, as well as to quantify gene expression by RT-qPCR. Type I and III collagen were quantified in the different sections by using an informatic plugin.

Resultados:

Mice treated with galvanic current presented an increase in polymorphonuclear cells and macrophages count 3 days after, with a parallel upregulation of Il6, Il1b, Cxcl10 and Il1ra gene expression. Nlrp3-/- mice had decreased expression of Il1b, Cxcl10, Il1ra and Tgfb compared to wild-type. In addition, galvanic current was able to increase collagen type I after 3 and 7 days in wild-type, but not in Nlrp3-/- mice. Tendon cellularity and collagen fibbers were not changing between needling and galvanic current.

Conclusiones:

Overall, we found that galvanic current could improve tendon regeneration by inducing an NLRP3-dependent inflammatory response and type I collagen increase. This effect might be mediated by TGF-, as gene expression was decreased in Nlrp3-/- mice.


Dirección

Campus de Ciencias de la Salud
Carretera Buenavista s/n, 30120 El Palmar
Murcia, España

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