Comunicación

EFFECT OF PERIPHERAL INFLAMMATION ON NEUROINFLAMMATORY RESPONSE OF THE RETINA

Autores:

Elsa Albero Ros1, Samuel David2, Manuel Vidal Sanz3, Marcelino Avilés Trigueros3

Afiliaciones:

(1) Universidad de Murcia, 30120, España (Región de Murcia)
(2) McGill University, Canadá
(3) OFTALMOLOGÍA EXPERIMENTAL, IMIB-Arrixaca, España

Comunicación:

Antecedentes:

To study how a peripheral inflammation process, caused by a well-established and characterized experimental model of polyarthritic inflammatory reaction in the articulations induced by Freund's complete adjuvant (FCA), promote the participation of the mononuclear phagocytic system in the neuroinflammatory response at the retinal level.

Métodos:

In adult C57BL/6J heterozygote lysozyme M-eGFP-knock-in (LysM-eGFP) mice, that express LysM-eGFP selectively in bone marrow myelomonocytic cells, a local unilateral arthritic inflammatory process was induced by intra-plantar FCA administration in the hind limb. Control groups were established with either saline treated (sham) or untreated (naive) animals. At different survival intervals (3, 7, and 10 days) FCA treated, sham or naive mice were processed. For each animal, both retinas were processed as whole-mounts and analysed immunohistologically for the expression of microglia markers and LysM-eGFP in mature myelomonocytic cells. Qualitative and quantitative changes in the distribution pattern and appearance of microglia and monocyte-derived macrophages were studied.

Resultados:

The eGFP-expressing (eGFP+) cell population in the innermost layer of the retina showed morphological signs compatible with microglia activation as early as 3 days after the CFA challenge and persisted throughout the time course of study (Figure 1.A-D). Morphologically, three subpopulations of eGFP+ cells were identified, amoeboid, ramified and peripheral shaped, which showed significant and relevant changes in its distribution pattern throughout the retina with respect to that observed in untreated retinas of sham or naive mice (Figure 1.E). Quantitative analysis of the eGFP+ and Iba-1 immunonegative amoeboid cell population showed a constant and similar number of cells to those counted in the sham or naive retinas over the time course. In contrast, in the first stage after the administration of FCA (3 dpl) there is a statistically significant depletion of eGFP+ and Iba-1 immunopositive (Iba-1+) amoeboid cells to reach normal values at later time points (7 and 10 dpl). However, the number of eGFP+ and Iba-1+ ramified-shaped cells reached its peak at 7 days after the lesion and then decreased to normal values. The number of eGFP+ and Iba-1+ perivascular cells was constant and similar in all study groups and along the time course.

Conclusiones:

In the retina of an adult mammal, a population of mononuclear phagocytic cells derived from bone marrow hematopoietic stem cells migrates and differentiates into microglia. The population of mononuclear phagocytic cells of the retina reacts to the induction of an arthritic inflammatory process through the administration of FCA, showing important qualitative and quantitative changes in response to the induced peripheral inflammatory process.


Image 1

Dirección

Campus de Ciencias de la Salud
Carretera Buenavista s/n, 30120 El Palmar
Murcia, España

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