Comunicación

ANALYSIS OF THE BODY COMPOSITION THROUGH COMPUTERIZED TOMOGRAPHY AFTER A FORCED WHEEL TRAINING PROGRAM IN MALE AND FEMALE ADOLESCENT RATS.

Autores:

Yevheniy Kutsenko1, Ángel Toval2, Daniel Garrigós2, Alberto Barreda3, Marta Martínez2, BRUNO RODOLFO RIBEIRO DO COUTO4, Jose Luis Ferrán Bertone5

Afiliaciones:

(1) Universidad de Murcia, Facultad de Medicina, 30006, Ucrania
(2) Universidad de Murcia, Facultad de Medicina, España (Región de Murcia)
(3) Laboratorios de Investigación Biomédica, España (Región de Murcia)
(4) PSICOBIOLOGÍA Y NEUROBIOLOGÍA DEL COMPORTAMIENTO, IMIB-Arrixaca, España
(5) REGIONALIZACION CEREBRAL Y GENES DEL DESARROLLO, IMIB-Arrixaca, España

Comunicación:

Antecedentes:

Overweight and obesity are defined by the World’s Health Organization as “abnormal or excessive fat accumulation that presents a risk to health” and they affect about the 25% of the globe’s population (WHO, 2020). The most effective approach to fight obesity is a change in nutritional and physical activity habits that derives in a negative energy balance (Petridou, Siopi, & Mougios, 2019). Physical activity (PA) stimulates the central nervous system (CNS), improving cognitive function, cardiovascular system, insulin sensitivity and glycemic control, reducing inflammatory markers and producing lipolysis (Saxton, Withers, & Heagerty, 2019; Tsai et al., 2019). Nonetheless, the CNS mechanisms through which physical activity regulates the adipose tissue (AT) contents have been practically unexplored. With the aim to follow CNS causal studies that lead to a change in AT contents due to physical activity, two aspects are essential: 1) Define precise tools to measure adipose, bone and lean tissue content during different periods of life and 2) develop an appropriate training program with effect in the body composition.

Métodos:

On postnatal day 30, 60 and 90 of the female and male baseline groups and postnatal day 27 and 45 of the male trained and male control groups, the rats were screened using an Albira trimodal preclinical-scanner and software (Fig 2). The 3D image segmentation was performed using the pMod version 3.5. From postnatal day 26, animals of the experimental group were exposed to an 8-days exercise habituation protocol in a forced running wheel system (Campden Instruments, 80805A) as described previously (A. Toval et al., 2017; Angel Toval et al., 2020). Each session, the rats were weighted. Details of the exercise duration and speed are described in Fig 1.

Resultados:

Female and male rats showed similar weights on postnatal day 30, but females showed higher thoracoabdominal (TA) volume. Nonetheless, P60 males showed higher weight and TA volume, being this difference more pronounced on P90. Between P30 and P90, the highest weight and volume gain and percentual increment was observed in males. Furthermore, male showed higher weight gain and percentual weight increment than female during adolescence (P30 to P60), and during young adulthood (P60 to P90). However, both females and males showed the highest percentual increment of weight and volume during adolescence (P30 to P60). The difference between the P30-Lean Tissue Content (LTC) and the P60-LTC is superior to the one between the P60-LTC and P90-LTC in both females and males. Regarding the bone tissue content (BTC), males showed higher values on P30 and higher BTC gain and percentual increment from P30 to P90 and from P30 to P60, but not from P60 to P90. After a forced wheel exercise program, experimental rats showed lower ATC and higher LTC and bone content.

Conclusiones:

Female and male SD rats experimented the highest gain in weight and volume during adolescence. Female SD rats gained more visceral adipose tissue, but males gained more subcutaneous during adolescence. Female and male SD rats showed the highest loss of relative lean and bone content during adolescence. This loss of lean and bone mass was prevented with forced wheel physical exercise during adolescence. Computerized tomography might be an appropriate method of analysis to detect small changes in body composition.


Image 1 Image 2

Dirección

Campus de Ciencias de la Salud
Carretera Buenavista s/n, 30120 El Palmar
Murcia, España

Ver en OpenStreetMap

Ver en Google Maps

Teléfonos

+34 868885229
+34 868885239
+34 868885249